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MicroRNAs (miRNAs) are small RNAs that post-transcription regulate the expression of thousands of genes in a broad range of organisms in both normal physiological contexts and in disease contexts..
miRNA expression profiling is gaining popularity as key regulators in gene expression networks, can influence many biological processes and also show promise as bio markers for disease..
Technological advances have spawned a multitude of platforms for miRNA profiling, and an understanding of the strengths and pitfalls of different approaches can aid in their effective use...
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miRNA Profiling | Thermo Fisher Scientific - UK

High Resolution Melting (HRM) analysis is a relatively new, post-PCR analysis method used to identify variations in nucleic acid sequences. The method is based on detecting small differences in PCR melting (dissociation) curves. It is enabled by imp